Human CRP (C-Reactive Protein) ELISA Kit - E-EL-H0043

Human CRP (C-Reactive Protein) ELISA Kit

The Human CRP ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CRP. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for CRP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of CRP. You can calculate the concentration of CRP in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: C-Reactive Protein

Abbreviation : CRP

Uniprot ID : P02741

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.469 ng/ml

Detection Range : 0.781 - 50 ng/ml

Manual & Datasheet

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