Human BBOX1 / Gamma-BBH Protein (His & GST tag) – PKSH030526

Recombinant Human BBOX1 / Gamma-BBH Protein (His & GST tag)

BBOX1, also known as gamma-BBH, belongs to the gamma-BBH/TMLD family. It is highly expressed in kidney and moderately expressed in liver. BBOX1 catalyzes the formation of L-carnitine from gamma-butyrobetaine, the last step in the L-carnitine biosynthetic pathway. Carnitine is essential for the transport of activated fatty acids across the mitochondrial membrane during mitochondrial beta-oxidation. BBOX1 is an inhibition target for mildronate which can be used to treat angina andmyocardial infarction. Mildronate may also be beneficial for the treatment ofneurological disorder, diabetes, and seizures and alcohol intoxication.

Synonym(s) : BBOX1 , BBH , BBOX

Species(s) : Human

Protein Expression Host : Baculovirus-Insect Cells

Protein Construction : A DNA sequence encoding the human BBOX1 (O75936) (Met1-Asn387) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Predicted N-terminal amino acid : Met

Molecular weight/mass : The recombinant human BBOX1 /GST chimera consists of 624 amino acids and has a calculated molecular mass of 72.5 kDa. The recombinant protein migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the human BBOX1 (O75936) (Met1-Asn387) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Formulation : Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerolcerol, pH 8.0

Purity : > 85 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet