Human BAFF/CD257 (B-Cell Activating Factor) ELISA Kit
The Human BAFF/CD257 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to BAFF/CD257. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for BAFF/CD257 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BAFF/CD257, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of BAFF/CD257. You can calculate the concentration of BAFF/CD257 in the samples by comparing the OD of the samples to the standard curve.
Detected Target name: B-Cell Activating Factor
Abbreviation : BAFF/CD257
Uniprot ID : Q9Y275
Format : 96 rx
Reactivity : Human
ELISA configuration : Sandwich
Sensitivity : 37.5 pg/ml
Detection Range : 62.5 – 4000 pg/ml
More information about this Human BAFF/CD257 (B-Cell Activating Factor) ELISA Kit from Elabscience
Additional information
Format | 96 rx |
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