Human B7-DC / PD-L2 / CD273 Protein (His & Fc tag) – PKSH031705

Recombinant Human B7-DC / PD-L2 / CD273 Protein (His & Fc tag)

Programmed death ligand 2 (PD-L2), also referred to as B7-DC and CD273, is a member of the B7 family of proteins including B7-1, B7-2, B7-H2, B7-H1 (PD-L1), and B7-H3. PD-L2 is a type I membrane protein and structurally consists of an extracellular region containing one V-like and one C-like Ig domain, a transmembrane region, and a short cytoplasmic domain. PD-L2 is expressed on antigen presenting cells, placental endothelium and medullary thymic epithelial cells, and can be induced by LPS in B cells, INF-γ in monocytes, or LPS plus IFN-γ in dendritic cells. The CD28 and B7 protein families are critical regulators of immune responses. PD-L2 and PD-L1 are two ligands for PD-1, member of the CD28/CTLA4 family expressed on activated lymphoid cells, and thus provide signals for regulating T cell activation and immune tolerance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity compared with the interaction of B7-H1/PD-1.

Synonym(s) : PDCD1LG2 , B7DC , Btdc , PDL2 , CD273 , PD-L2 , PDCD1L2 , MGC142238 , MGC142240 , bA574F11.2

Species(s) : Human

Protein Expression Host : Human Cells

Protein Construction : A DNA sequence encoding the extracellular domain (Met 1-Pro 219) of human PD-L2 (NP_079515.2) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus.

Predicted N-terminal amino acid : Leu 20

Molecular weight/mass : The recombinant human PD-L2/Fc is a disulfide-linked homodimeric protein after proteolytic removal of the signal peptide. The reduced monomer consists of 447 amino acids and predicts a molecular mass of 50.4 kDa. As a result of glycosylation, the apparent molecular mass of rh PD-L2/Fc monomer is approximately 70-80 kDa in SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the extracellular domain (Met 1-Pro 219) of human PD-L2 (NP_079515.2) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus.

Formulation : Lyophilized from sterile PBS, pH 7.4

Purity : > 97 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet