Human B2M / Beta-2-microglobulin Protein (His tag) – PKSH030949

Recombinant Human B2M / Beta-2-microglobulin Protein (His tag)

B2M, also known as β2-Microglobulin or CDABP0092, is a component of MHC class I molecules found expression in all nucleated cells (excludes red blood cells). The major function of MHC class I moleculesis is to display fragments of proteins from within the cell to T-cells and cells containing foreign proteins will be attacked. B2M(β2-Microglobulin) is a low molecular weight protein. It was demonstrated that B2M(β2-Microglobulin) was localized in the membranes of nucleated cells and was found to be associated with HL-A antigens.B2M(β2- Microglobulin) is present in free form in various body fluids and as a subunit of histocompatibility antigens on cell surfaces lateral to theα3 chain. Unlikeα3, β2 has no transmembrane region. Directly above β2 lies the α1 chain, which itself is lateral to the α2. In the absence of B2M(β2 microglobulin), very limited amounts of MHC class I (classical and non-classical) molecules can be detected on the surface. In the absence of MHC class I, CD8 T cells, a subset of T cells involved in the development of acquired immunity cannot develop. Low levels of B2M(β2 microglobulin) can indicate non-progression of HIV.

Synonym(s) : CDABP0092

Species(s) : Human

Protein Expression Host : Human Cells

Protein Construction : A DNA sequence encoding the human B2M (NP_004039.1) (Met 1-Met 119) was expressed, with a polyhistidine tag at the C-terminus.

Predicted N-terminal amino acid : Ile 21

Molecular weight/mass : The recombinant human B2M consists of 110 amino acids and migrates as an approximately 13.5 kDa band in SDS-PAGE under reducing conditions as predicted.

Protein Construction : A DNA sequence encoding the human B2M (NP_004039.1) (Met 1-Met 119) was expressed, with a polyhistidine tag at the C-terminus.

Formulation : Lyophilized from sterile PBS, pH 7.4

Purity : > 97 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet