Human Autotaxin / ENPP2 / NPP2 Protein (His tag) – PKSH031157

Recombinant Human Autotaxin / ENPP2 / NPP2 Protein (His tag)

ENPP2 (Ectonucleotide pyrophosphatase/phosphodiesterase family member 2), also referred as Autotaxin, is a secreted enzyme encoded by the ENPP2 gene. This gene product stimulates the motility of tumor cells, has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The Autotaxin protein is important for generating the lipid signaling molecule lysophosphatidic acid (LPA), which is a potent mitogen, which facilitates cell proliferation and migration, neurite retraction, platelet aggregation, smooth muscle contraction, actin stress formation and cytokine and chemokine secretion. ATX has been found to catalyze the formation of cyclic phosphatidic acid (cPA), which have antitumor role by antimitogenic regulation of cell cycle, inhibition of cancer invasion and metastasis. LPA receptors and ATX are upregulated in numerous cancer cell types and show expression patterns that correlate with tumor cell invasiveness. Thus, Autotaxin has recently emerged as an attractive target for the development of anti-cancer chemotherapeutics. In addition, Serum ATX activity was found to be enhanced in relation to hepatic fibrosis in chronic liver disease due to hepatitis virus C infection.

Synonym(s) : ATX , ATX-X , AUTOTAXIN , LysoPLD , NPP2 , PD-IALPHA , PDNP2

Species(s) : Human

Protein Expression Host : Human Cells

Protein Construction : A DNA sequence encoding the mature form of human ENPP2 (AAH34961.1) (Asp 49-Ile 863) was expressed, with a polyhistidine tag at the N-terminus.

Predicted N-terminal amino acid : His

Molecular weight/mass : The recombinant human ENPP2 consists of 834 amino acids and has a calculated molecular mass of 96 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhENPP2 is approximately 120-130 kDa due to glycosylation.

Protein Construction : A DNA sequence encoding the mature form of human ENPP2 (AAH34961.1) (Asp 49-Ile 863) was expressed, with a polyhistidine tag at the N-terminus.

Formulation : Lyophilized from sterile PBS, pH 7.4

Purity : > 88 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet