Human ARG1 / Arginase 1 Protein (His & MYC Tag) – PKSH030301

Recombinant Human ARG1 / Arginase 1 Protein (His & MYC Tag)

Arginase is the focal enzyme of the urea cycle hydrolysing L-arginine to urea and L-ornithine. Emerging studies have identified arginase in the vasculature and have implicated this enzyme in the regulation of nitric oxide (NO) synthesis and the development of vascular disease. Arginase also redirects the metabolism of L-arginine to L-ornithine and the formation of polyamines and L-proline, which are essential for smooth muscle cell growth and collagen synthesis. Arginase is encoded by two recently discovered genes (Arginase I and Arginase II). In most mammals, Arginase 1 (ARG1) also known as Arginase, liver, which functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. It is located in mitochondria of several tissues in the body, with most abundance in the kidney and prostate. It may be found at lower levels in macrophages, lactating mammary glands, and brain.

Synonym(s) : ARG1

Species(s) : Human

Protein Expression Host : Human Cells

Protein Construction : A DNA sequence encoding the human ARG1 (NP_000036.2) (Met1-Lys322) was expressed with a polyhistidine tag at the N-terminus and a myc tag at the C-terminus.

Predicted N-terminal amino acid : His

Molecular weight/mass : The recombinant human ARG1 consists of 338 amino acids and predicts a molecular mass of 36.7 kDa.

Protein Construction : A DNA sequence encoding the human ARG1 (NP_000036.2) (Met1-Lys322) was expressed with a polyhistidine tag at the N-terminus and a myc tag at the C-terminus.

Formulation : Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 20 % glycerol, pH 7.4

Purity : > 95 % as determined by SDS-PAGE.

Endotoxin level : < 1.0 EU per μg protein as determined by the LAL method.

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet