Human ApoA4 (Apolipoprotein A4) ELISA Kit – E-EL-H0463

Human ApoA4 (Apolipoprotein A4) ELISA Kit

The Human ApoA4 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ApoA4. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for ApoA4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ApoA4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ApoA4. You can calculate the concentration of ApoA4 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Apolipoprotein A4

Abbreviation : ApoA4

Uniprot ID : P06727

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 2.813 ng/ml

Detection Range : 4.688 – 300 ng/ml

Manual & Datasheet

More information about this Human ApoA4 (Apolipoprotein A4) ELISA Kit from Elabscience