Human Ang-III (Angiotensin III) ELISA Kit – E-EL-H0354

Human Ang-III (Angiotensin III) ELISA Kit

The Human Ang-III ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with Human Ang-III. During the reaction, Human Ang-III in the sample or standard competes with a fixed amount of Human Ang-III on the solid phase supporterfor sites on the Biotinylated Detection Ab specific to Human Ang-III. Excess conjugate and unbound sample or standard are washed from the plate, andAvidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Ang-III in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: Angiotensin III

Abbreviation : Ang-III

Uniprot ID :

Format : 96 rx

Reactivity : Human

ELISA configuration : Competitive

Sensitivity : 46.875 pg/ml

Detection Range : 78.125 – 5000 pg/ml

Manual & Datasheet

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