Human AMBP (Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit
The Human AMBP/α1M ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AMBP/α1M. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for AMBP/α1M and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AMBP/α1M, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of AMBP/α1M. You can calculate the concentration of AMBP/α1M in the samples by comparing the OD of the samples to the standard curve.
Detected Target name: Alpha-1-Microglobulin/Bikunin Precursor
Abbreviation : AMBP
Uniprot ID : P02760
Format : 96 rx
Reactivity : Human
ELISA configuration : Sandwich
Sensitivity : 2.813 ng/ml
Detection Range : 4.688 – 300 ng/ml
Additional information
Format | 96 rx |
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