Human AKT1 / PKB / PKBα Protein (His tag) – PKSH031439

Recombinant Human AKT1 / PKB / PKBα Protein (His tag)

v-akt murine thymoma viral oncogene homolog 1 (AKT1), or protein kinase B-alpha (PKB-ALPHA) is a serine-threonine protein kinase, belonging to the Protein Kinase Superfamily. AKT1 is a major mediator of the responses to insulin, insulin-like growth factor 1 (IGF1), and glucose. AKT1 also plays a key role in the regulation of both muscle cell hypertrophy and atrophy. AKT1 activity is required for physiologic cardiac growth in response to IGF1 stimulation or exercise training. In contrast, AKT1 activity was found to antagonize pathologic cardiac growth that occurs in response to endothelin 1 stimulation or pressure overload. AKT1 selectively promotes physiological cardiac growth while AKT2 selectively promotes insulin-stimulated cardiac glucose metabolism. AKT1 deletion prevented tumor initiation as well as tumor progression, coincident with decreased Akt signaling in tumor tissues. AKT1 is the primary Akt isoform activated by mutant K-ras in lung tumors, and that AKT3 may oppose AKT1 in lung tumorigenesis and lung tumor progression. A number of separate studies have implicated AKT1 as an inhibitor of breast epithelial cell motility and invasion. AKT1 may have a dual role in tumorigenesis, acting not only pro-oncogenically by suppressing apoptosis but also anti-oncogenically by suppressing invasion and metastasis.

Synonym(s) : AKT1 , AKT1 , AKT , MGC99656 , PKB , PKB-ALPHA , PRKBA , RAC , RAC-ALPHA

Species(s) : Human

Protein Expression Host : Baculovirus-Insect Cells

Protein Construction : A DNA sequence encoding the human AKT1 (NP_001014431.1) (Met 1-Ala 480) was fused with a polyhistidine tag at the C-terminus.

Predicted N-terminal amino acid : Met 1

Molecular weight/mass : The recombinant human AKT1 consists of 491 amino acids and migrates as an approximately 57 kDa band as predicted in SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the human AKT1 (NP_001014431.1) (Met 1-Ala 480) was fused with a polyhistidine tag at the C-terminus.

Formulation : Lyophilized from sterile 50mM Tris, 100mM NaCl, 3mM DTT, 0.5mM PMSF, 5% Glycerol, pH 8.0

Purity : > 87 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet