Human AgRP Protein (Fc tag) – PKSH031895

Recombinant Human AgRP Protein (Fc tag)

Agouti Related Protein (AGRP, or AGRT), is an endogenous antagonist of the melanocortin receptors MC3R and MC4R found in the hypothalamus and exhibits potent orexigenic activity. AGRP can act as a competitive antagonist to proopiomelanocortin (POMC)-derived peptides at the melanocortin-4 receptor (MC4R), and that this homeostatic mechanism is important as a means of coordinating appetite with perceived metabolic requirement. AGRP is upregulated by fasting while intracerebroventricular injections of synthetic AGRP lead to increased appetite and food intake. Thus, AGRP is a powerful orexigenic peptide that increases food intake when ubiquitously overexpressed or when administered centrally.

Synonym(s) : AGRP , AGRT , ART , ASIP2 , MGC118963

Species(s) : Human

Protein Expression Host : Human Cells

Protein Construction : A DNA sequence encoding the human AgRP (NP_001129.1) (Met 1-Thr 132) was expressed with the fused Fc region of human IgG1 at the C-terminus.

Predicted N-terminal amino acid : Ala 21

Molecular weight/mass : The recombinant human AgRP/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 350 amino acids and predicts a molecular mass of 39 kDa also estimated by SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the human AgRP (NP_001129.1) (Met 1-Thr 132) was expressed with the fused Fc region of human IgG1 at the C-terminus.

Formulation : Lyophilized from sterile PBS, pH 7.2

Purity : > 97 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet