Human AFP / alpha-fetoprotein Protein (His tag) – PKSH030882

Recombinant Human AFP / alpha-fetoprotein Protein (His tag)

Alpha-fetoprotein (AFP) is classified as a member of the albuminoid gene superfamily consisting of albumin, AFP, vitamin D (Gc) protein, and alpha-albumin. AFP is a glycoprotein of 591 amino acids and a carbohydrate moiety. AFP is one of the several embryo-specific proteins and is a dominant serum protein as early in human embryonic life as one month, when albumin and transferrin are present in relatively small amounts. It is first synthesized in the human by the yolk sac and liver(1-2 months) and subsequently predominantly in the liver. A small amount of AFP is produced by the GI tract of the human conceptus. It has been proved that AFP may reappear in the serum in elevated amounts in adult life in association with normal restorative processes and with malignant growth. Alpha-fetoprotein (AFP) is a specific marker for hepatocellular carcinoma (HCC), teratoblastomas, and neural tube defect (NTD).

Synonym(s) : FETA , HPAFP

Species(s) : Human

Protein Expression Host : Human Cells

Protein Construction : A DNA sequence encoding the human AFP (P02771) (Met 1-Val 609) was fused with a polyhistidine tag at the C-terminus.

Predicted N-terminal amino acid : Arg 19

Molecular weight/mass : The recombinant human AFP consists of 602 amino acids and has a predicted molecular mass of 67.9 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhAFP is approximately 65 kDa.

Protein Construction : A DNA sequence encoding the human AFP (P02771) (Met 1-Val 609) was fused with a polyhistidine tag at the C-terminus.

Formulation : Lyophilized from sterile PBS, pH 7.4

Purity : > 97 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet