Human ACPL2 Protein (His tag) – PKSH031183

Recombinant Human ACPL2 Protein (His tag)

acid phosphatase-like protein 2, also known as ACPL2, is a secreted protein which belongs to the histidine acid phosphatase family. A large-scale effort, termed the Secreted Protein Discovery Initiative (SPDI), was undertaken to identify novel secreted and transmembrane proteins. In the first of several approaches, a biological signal sequence trap in yeast cells was utilized to identify cDNA clones encoding putative secreted proteins. A second strategy utilized various algorithms that recognize features such as the hydrophobic properties of signal sequences to identify putative proteins encoded by expressed sequence tags (ESTs) from human cDNA libraries. A third approach surveyed ESTs for protein sequence similarity to a set of known receptors and their ligands with the BLAST algorithm. Finally, both signal-sequence prediction algorithms and BLAST were used to identify single exons of potential genes from within human genomic sequence.

Synonym(s) : ACPL2 , UNQ370/PRO706 , FLJ23751

Species(s) : Human

Protein Expression Host : Human Cells

Protein Construction : A DNA sequence encoding the human ACPL2 (NP_689495.1) (Met 1-Phe 480) precursor was fused with a polyhistidine tag at the C-terminus.

Predicted N-terminal amino acid : Leu 24

Molecular weight/mass : The recombinant human ACPL2 consists of 468 amino acids after removal of the signal peptide and has a predicted molecular mass of 54 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the human ACPL2 (NP_689495.1) (Met 1-Phe 480) precursor was fused with a polyhistidine tag at the C-terminus.

Formulation : Lyophilized from sterile PBS, pH 7.4

Purity : > 95 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet