FlyCut™ Sma I – JS501

FlyCut™ Sma I Restriction Endonuclease

FlyCut™ Sma I is expressed and purified from E.coli that carries the recombinant Sma I gene. The molecular weight is 29.7 kDa, with the recognition site is CCC^GGG. The optimal reaction temperature is 25°C for full activity or 37°C for 50% activity. Heat inactivation can be achieved by incubating at 65°C for 20 minutes. This enzyme is not sensitive to dam or dcm methylation but is sensitive to mammalian CpG methylation.

Enzyme Properties

• Fast cleavage in 5-10 minutes with high fidelity
• 20 000 u/ml

FlyCut™ Sma I components

• FlyCut™ Sma I : 1 000 u / 2 x 1 000 u
• 10x FlyCut™ Buffer: 250 ul / 500 ul
• 10x DNA Loading Buffer: 1 ml / 1 ml

Manual & Datasheet

JS501 – FlyCut Sma I