Chicken IFN-α (Interferon Alpha) ELISA Kit – E-EL-Ch0130

Chicken IFN-α (Interferon Alpha) ELISA Kit

The Chicken IFN-α ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IFN-α. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for IFN-α and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IFN-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of IFN-α. You can calculate the concentration of IFN-α in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Chicken Interferon Alpha

Abbreviation : IFN-α

Uniprot ID : Q38IF1

Format : 96 rx

Reactivity : Chicken

ELISA configuration : Sandwich

Sensitivity : 9.375 pg/ml

Detection Range : 15.625 – 1000 pg/ml

Manual & Datasheet

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