Chicken HSP-70/HSPA9 (Heat Shock Protein 70) ELISA Kit – E-EL-Ch1706

Chicken HSP-70/HSPA9 (Heat Shock Protein 70) ELISA Kit

The Chicken HSP-70/HSPA9 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HSP-70/HSPA9. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for HSP-70/HSPA9 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HSP-70/HSPA9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of HSP-70/HSPA9. You can calculate the concentration of HSP-70/HSPA9 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Chicken Heat Shock Protein 70

Abbreviation : HSP-70/HSPA9

Uniprot ID : Q7SX63

Format : 96 rx

Reactivity : Chicken

ELISA configuration : Sandwich

Sensitivity : 0.469 ng/ml

Detection Range : 0.781 – 50 ng/ml

Manual & Datasheet

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