Chicken FSH (Follicle-Stimulating Hormone) ELISA Kit – E-EL-Ch1365

Chicken FSH (Follicle-Stimulating Hormone) ELISA Kit

The Chicken FSH ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to FSH. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for FSH and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FSH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of FSH. You can calculate the concentration of FSH in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Chicken Follicle-Stimulating Hormone

Abbreviation : FSH

Uniprot ID :

Format : 96 rx

Reactivity : Chicken

ELISA configuration : Sandwich

Sensitivity : 9.375 pg/ml

Detection Range : 15.625 – 1000 pg/ml

Manual & Datasheet

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