Genotyping by PCR of a Large Colony of Mice

Project Description

Mouse Genotyping by PCR of a large mice colony

Mouse Genotyping by PCR works well in their lab, but costs 1000$ for 1000 units of Titanium Taq 🙁

This project illustrates one of the main reasons why we offer FREE PCR services:

Researchers don’t like to test Taq samples for many reasons:

• too many suppliers;
• they all say their Taq will work;
• it requires time to perform tests and performance comparison.

This well-known researcher who’s lab is located at McGill’s Goodman Research Center in Montreal had been using a really expensive Taq DNA Polymerase for many years. But now is the time for change. We offered them our free PCR service. They provided us with protocols, DNA primers and mouse tail gDNA samples (not purified). Please find bellow what we have accomplished using Transgen Biotech’s TransStart TopTaq DNA Polymerase. Because this Mouse Genotyping by PCR project was large, we will not provide PCR setup and cycling protocols for all genes and for simplicity reasons, we will not provide all primer sequences.

The cost for TransStart TopTaq DNA Polymerase currently is 894$ for 3000 units, thereby reducing the researchers cost for genotyping his mice colony by more than 66% without affecting the reliability of their PCR experiments.