VB1 (Vitamin B1) ELISA Kit – E-EL-0007

VB1 (Vitamin B1) ELISA Kit

The VB1 ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with VB1. During the reaction, VB1 in the sample or standard competes with a fixed amount of VB1 on the solid phase supporterfor sites on the Biotinylated Detection Ab specific to VB1. Excess conjugate and unbound sample or standard are washed from the plate, andAvidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VB1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: VB1

Abbreviation : Thiamine, Thiamin, Aneurine Hydrochloride, thio-vitamine

Uniprot ID :

Format : 96 rx

Reactivity : Universal (All Species expexted)

ELISA configuration : Competitive

Sensitivity : 2,813

Detection Range : 4.688-300 ng/mL

More information about this VB1 (Vitamin B1) ELISA Kit from Elabscience