SPlink Detection Kit (ABC for IHC)

Streptavidin is a protein that has similar binding properties to avidin.It is isolated from streptomyces avidinii. Streptavidin has a molecular weight of 60 KD and has 4 subunits. Each subunit can bind one molecule of biotin.SPlink Detection Kit is coupled steptavidin with horseradish peroxidase (HRP). This highly sensitive detector conjugate binds to biotinylated secondary antibodies in immunohistochemistry.

Components

IHC Detection Procedure

1. Section paraffin,dewax conventionally

2. Antigen retrieval (this step is optional according to the experiment requirement)

3. Incubate with 3% H2O2 deionized water for 5~10min to eliminate endogenous peroxidase activity.

4. Wash with PBS, 3 min, 3 times

5. Add reagent A, incubate at room temperature for 10~15min, abandon the reagent, don’t wash;

6. Add primary antibody with proper dilution ratio,incubate at 37℃ for 2~3h or at 4℃ for one night

7. Wash with PBS, 3 min, 3 times

8. Add reagent B, incubate at 37℃ or room temperature for 10~15min

9. Wash with PBS, 3 min, 3 times

10. Add reagent C, incubate at 37℃ or room temperature for 10~15min

11. Wash with PBS, 3 min, 3 times

12. Perform chromogenic reaction with chromogenic reagent (DAB or ACE)

13. Wash with tap water

14. Make the section counterstained,dewatered, transparent if the experiment requires

15. Mount the section with proper mounting medium

Storage

Stored at 2°C – 8°C. Shading light.

Manual and Datasheet