Rat VMA (Vanillylmandelic Acid) ELISA Kit – E-EL-R2511

Rat VMA (Vanillylmandelic Acid) ELISA Kit

The VMA ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with Rat VMA. During the reaction, Rat VMA in the sample or standard competes with a fixed amount of Rat VMA on the solid phase supporterfor sites on the Biotinylated Detection Ab specific to Rat VMA. Excess conjugate and unbound sample or standard are washed from the plate, andAvidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat VMA in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: Rat Vanillylmandelic Acid

Abbreviation : VMA

Uniprot ID :

Format : 96 rx

Reactivity : Rat

ELISA configuration : Competitive

Sensitivity : 1.875 ng/ml

Detection Range : 3.125 – 200 ng/ml

Manual & Datasheet

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