Rat VIP (Vasoactive Intestinal Peptide) ELISA Kit – E-EL-R1062

Rat VIP (Vasoactive Intestinal Peptide) ELISA Kit

The VIP ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with Rat VIP. During the reaction, Rat VIP in the sample or standard competes with a fixed amount of Rat VIP on the solid phase supporterfor sites on the Biotinylated Detection Ab specific to Rat VIP. Excess conjugate and unbound sample or standard are washed from the plate, andAvidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat VIP in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: Rat Vasoactive Intestinal Peptide

Abbreviation : VIP

Uniprot ID : P01283

Format : 96 rx

Reactivity : Rat

ELISA configuration : Competitive

Sensitivity : 4.688 pg/ml

Detection Range : 7.813 – 500 pg/ml

Manual & Datasheet

More information about this Rat VIP (Vasoactive Intestinal Peptide) ELISA Kit from Elabscience