Rat UCHL3 Protein (His tag) – PKSR030368

Recombinant Rat UCHL3 Protein (His tag)

Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCH-L3, Ubiquitin thioesterase L3 and UCHL3, is a ubiquitin-protein hydrolase which belongs to the peptidase C12 family. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCHL3 is highly expressed in heart, skeletal muscle, and testis. UCHL1 and UCHL3 are two of the deubiquitinating enzymes expressed in the brain. These phenotypes indicate the importance of UCHL1 and UCHL3 in the regulation of the central nervous system. UCHL3 functions as a de-ubiquitinating enzyme where lack of its hydrolase activity may result in the prominent accumulation of ubiquitinated proteins and subsequent induction of stress responses in skeletal muscle. UCHL3 has also been identified as a tumor-specific antigen in colon cancer.

Synonym(s) : rCG_37146 , RGD1561196

Species(s) : Rat

Protein Expression Host : E. coli

Protein Construction : A DNA sequence encoding the rat UCHL3 (Q91Y78) (Glu 2-Ala 230) was fused with a polyhistidine tag at the N-terminus.

Predicted N-terminal amino acid : Met

Molecular weight/mass : The recombinant rat UCHL3 comprises 240 amino acids and predicts a molecular mass of 27.5 kDa. The apparent molecular mass of the rat UCHL3 is approximately 28 kDa in SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the rat UCHL3 (Q91Y78) (Glu 2-Ala 230) was fused with a polyhistidine tag at the N-terminus.

Formulation : Lyophilized from sterile PBS, pH 7.4

Purity : > 95 % as determined by SDS-PAGE

Endotoxin level :

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet