Rat UCHL1 (Ubiquitin Carboxyl Terminal Hydrolase L1) ELISA Kit – E-EL-R2478

Rat UCHL1 (Ubiquitin Carboxyl Terminal Hydrolase L1) ELISA Kit

The UCHL1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat UCHL1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat UCHL1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat UCHL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat UCHL1. You can calculate the concentration of Rat UCHL1 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Ubiquitin Carboxyl Terminal Hydrolase L1

Abbreviation : UCHL1

Uniprot ID : Q00981

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 37.5 pg/ml

Detection Range : 62.5 – 4000 pg/ml

Manual & Datasheet

More information about this Rat UCHL1 (Ubiquitin Carboxyl Terminal Hydrolase L1) ELISA Kit from Elabscience