Rat TRF (Transferrin) ELISA Kit – E-EL-R1179

Rat TRF (Transferrin) ELISA Kit

The TF ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TRF. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for TRF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TRF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of TRF. You can calculate the concentration of TRF in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Transferrin

Abbreviation : TF

Uniprot ID : P12346

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 0.938 ng/ml

Detection Range : 1.563 – 100 ng/ml

Manual & Datasheet

More information about this Rat TRF (Transferrin) ELISA Kit from Elabscience