Rat TNX (Tenascin X) ELISA Kit – E-EL-R0949

Rat TNX (Tenascin X) ELISA Kit

The TNX ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TNX. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat TNX and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TNX, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TNX. You can calculate the concentration of Rat TNX in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Tenascin X

Abbreviation : TNX

Uniprot ID : Q9Z0U2

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 0.188 ng/ml

Detection Range : 0.313 – 20 ng/ml

Manual & Datasheet

More information about this Rat TNX (Tenascin X) ELISA Kit from Elabscience