Rat Tn-I (Troponin I) ELISA Kit – E-EL-R0055

Rat Tn-I (Troponin I) ELISA Kit

The Tn-I ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Tn-I. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Tn-I and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Tn-I, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Tn-I. You can calculate the concentration of Tn-I in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Troponin I

Abbreviation : Tn-I

Uniprot ID : P23693

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 9.375 pg/ml

Detection Range : 15.625 – 1000 pg/ml

Manual & Datasheet

More information about this Rat Tn-I (Troponin I) ELISA Kit from Elabscience