Rat TLR2 Protein (His tag) – PKSR030182

Recombinant Rat TLR2 Protein (His tag)

TLR2, also known as CD282, is a member of the Toll-like receptor (TLR) family. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They play a fundamental role in pathogen recognition and activation of innate immunity. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. TLR2 contains 14 LRR (leucine-rich) repeats and 1 TIR domain. TLR2 gene is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. CD282 cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. It also cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. CD282 acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. It may also promote apoptosis in response to lipoproteins.

Synonym(s) : TLR2

Species(s) : Rat

Protein Expression Host : Baculovirus-Insect Cells

Protein Construction : A DNA sequence encoding the mature form of rat TLR2 (Q6YGU2) (Met1-Gln587) was expressed,with a polyhistide tag at the C-terminus.

Predicted N-terminal amino acid : Leu 17

Molecular weight/mass : The recombinant rat TLR2 consists of 597 amino acids and predicts a molecular mass of 67.5 KDa. It migrates as an approximately 60 KDa band in SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the mature form of rat TLR2 (Q6YGU2) (Met1-Gln587) was expressed,with a polyhistide tag at the C-terminus.

Formulation : Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol

Purity : > 90 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet