Rat Pepsinogen C / PGC Protein (His tag) – PKSR030364

Recombinant Rat Pepsinogen C / PGC Protein (His tag)

Pepsinogen C, also known as PGC, is an aspartic proteinase that belongs to the peptidase family A1. Pepsinogen C is synthesized in the gastric mucosa as inactive precursors, known as zymogens. Pepsinogen C contains a prosegment that serves to stabilize the inactive form and prevent entry of the substrate to the active site. At low PH conditions, Pepsinogen C undergoes conversion into active enzyme. Pepsinogen C has been found expressed in all regions of the stomach mucosa and also in the proximal duodenal mucosa. In stomach cancer tissues and cancer cell lines, the expressions of the pepsinogen genes were decreased or lost, in good accordance with their pepsinogen productions. No gross structural changes of the pepsinogen genes were observed in these cancers, but the methylation patterns of the pepsinogen genes were found to be altered in different ways in different cancers. Serum levels of Pepsinogen C are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis.

Synonym(s) : PG1 , Pg-1 , Upg1

Species(s) : Rat

Protein Expression Host : Human Cells

Protein Construction : A DNA sequence encoding the rat PGC (NP_579818.1) (Met 1-Val 392) was expressed, fused with a polyhistidine tag at the C-terminus.

Predicted N-terminal amino acid : Ser 17

Molecular weight/mass : The recombinant rat PGC comprises 387 amino acids and predicts a molecular mass of 42.5 kDa. The apparent molecular mass of the ratPGC is approximately 43 kDa in SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the rat PGC (NP_579818.1) (Met 1-Val 392) was expressed, fused with a polyhistidine tag at the C-terminus.

Formulation : Lyophilized from sterile 50mM Tris, 150mM NaCl, pH 7.5

Purity : > 95 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet