Rat MDHA / MDH1 Protein (His tag) – PKSR030299

Recombinant Rat MDHA / MDH1 Protein (His tag)

Malate dehydrogenases 1(MDH1 / MDHA) is soluable form of malate dehydrogenases. Malate dehydrogenases (MDH) is a group of multimeric enzymes consisting of identical subunits usually organized as either dimer or tetramers with subunit molecular weights of 30-35 kDa. MDH has been isolated from different sources including archaea, eubacteria, fungi, plant and mammals. MDH catalyzes the NAD/NADH-dependent interconversion of the substrates malate and oxaloacetate. This reaction plays a key part in the malate / aspartate shuttle across the mitochondrial membrane, and in the tricarboxylic acid cycle within the mitochondrial matrix. The enzymes share a common catalytic mechanism and their kinetic properties are similar, which demonstrates a high degree of structural similarity. The three-dimensional structures and elements essential for catalysis are conserved between mitochondrial and cytoplasmic forms of MDH in eukaryotic cells even though these isoenzymes are only marginally related at the level of primary structure.

Synonym(s) : MDL1 , Mdhl , Mor2

Species(s) : Rat

Protein Expression Host : E. coli

Protein Construction : A DNA sequence encoding the rat MDH1 (O88989) (Met 4-Ala 334) was expressed with a polyhistidine tag at the C-terminus.

Predicted N-terminal amino acid : Met 1

Molecular weight/mass : The recombinant rat MDH1 comprises 345 amino acids and predicts a molecular mass of 38 kDa. The apparent molecular mass of the rat MDH1 is approximately 39 kDa in SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the rat MDH1 (O88989) (Met 4-Ala 334) was expressed with a polyhistidine tag at the C-terminus.

Formulation : Lyophilized from sterile 20mM Tris, 10% glycerolcerol, pH 8.0

Purity : > 90 % as determined by SDS-PAGE

Endotoxin level :

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet