Rat BNP (Brain Natriuretic Peptide) ELISA Kit – E-EL-R0126

Rat BNP (Brain Natriuretic Peptide) ELISA Kit

The BNP ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with BNP. During the reaction, Rat BNP in the sample or standard competes with a fixed amount of BNP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat BNP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat BNP in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: Rat Brain Natriuretic Peptide

Abbreviation : BNP

Uniprot ID : P13205

Format : 96 rx

Reactivity : Rat

ELISA configuration : Competitive

Sensitivity : 18.75 pg/ml

Detection Range : 31.25 – 2000 pg/ml

Manual & Datasheet

More information about this Rat BNP (Brain Natriuretic Peptide) ELISA Kit from Elabscience