Rat bFGF/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit – E-EL-R0091

Rat bFGF/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit

The bFGF/FGF2 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to bFGF/FGF2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for bFGF/FGF2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain bFGF/FGF2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of bFGF/FGF2. You can calculate the concentration of bFGF/FGF2 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Basic Fibroblast Growth Factor

Abbreviation : bFGF/FGF2

Uniprot ID : P13109

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 9.375 pg/ml

Detection Range : 15.625 – 1000 pg/ml

Manual & Datasheet

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