Rat ANGPTL3 (Angiopoietin Like Protein 3) ELISA Kit – E-EL-R2449

Rat ANGPTL3 (Angiopoietin Like Protein 3) ELISA Kit

The ANGPTL3 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ANGPTL3. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for ANGPTL3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ANGPTL3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ANGPTL3. You can calculate the concentration of ANGPTL3 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Angiopoietin Like Protein 3

Abbreviation : ANGPTL3

Uniprot ID : F7FHP0

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 37.5 pg/ml

Detection Range : 62.5 – 4000 pg/ml

Manual & Datasheet

More information about this Rat ANGPTL3 (Angiopoietin Like Protein 3) ELISA Kit from Elabscience