Rat AhR (Aryl Hydrocarbon Receptor) ELISA Kit – E-EL-R0072

Rat AhR (Aryl Hydrocarbon Receptor) ELISA Kit

The AhR ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AhR. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for AhR and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AhR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of AhR. You can calculate the concentration of AhR in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Aryl Hydrocarbon Receptor

Abbreviation : AhR

Uniprot ID : P41738

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 0.188 ng/ml

Detection Range : 0.313 – 20 ng/ml

Manual & Datasheet

More information about this Rat AhR (Aryl Hydrocarbon Receptor) ELISA Kit from Elabscience