Rat AFGF/FGF1 (Acidic Fibroblast Growth Factor 1) ELISA Kit – E-EL-R2407

Rat AFGF/FGF1 (Acidic Fibroblast Growth Factor 1) ELISA Kit

The AFGF/FGF1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AFGF/FGF1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for AFGF/FGF1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AFGF/FGF1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of AFGF/FGF1. You can calculate the concentration of AFGF/FGF1 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Acidic Fibroblast Growth Factor 1

Abbreviation : AFGF/FGF1

Uniprot ID : P61149

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 9.375 pg/ml

Detection Range : 15.625 – 1000 pg/ml

Manual & Datasheet

More information about this Rat AFGF/FGF1 (Acidic Fibroblast Growth Factor 1) ELISA Kit from Elabscience