ProteinExt® Mammalian Membrane Protein Extraction Kit

ProteinExt® Mammalian Membrane Protein Extraction Kit provides a simple and efficient way to isolate and extract membrane protein from mammalian cells and tissues. Native proteins can be obtained within 70 minutes without the ultracentrifugation. Up to 90% efficiency for membrane proteins with at least 1-2 transmembrane domains. The extracted protein is suitable for a variety of downstream applications, including SDS-PAGE, Western Blot, ELISA, and enzyme-activity assays.

Enough extraction reagent is provided for 50 x 0.5-2.0 E07 cells.

Kit Content:

• 50 ml Membrane Protein Extraction Buffer I (MPEB I)
• 7.5 ml Membrane Protein Extraction Buffer II (MPEB II)
• 15 ml Membrane Protein Extraction Buffer III (MPEB III)
• 1 ml ProteinSafe™ Protease Inhibitor Cocktail, EDTA-free (100x)

Manual and datasheet:

ProteinExt Mammalian Membrane Protein Extraction Kit