Proteinase K (20 mg/ml) – GE201-01

Proteinase K (20 mg/ml)

Proteinase K is a nonspecific serine protease that will hydrolyze a variety of peptide bands. Proteinase K is active in a broad range of temperature and buffers. It cannot be inactivated by metal ions, chelating agents (e.g. EDTA), or detergents such as SDS.

Properties of Proteinase K

• Active in a wide range of buffers and pH value.
• Incubation temperature: 55-65 °C
• Incubation time: 15 minutes to 48 hours; optimal incubation time: 2 hours
• Included in most of our genomic DNA extraction kits and RNA extraction kits

Source

• Purified from Tritirachium album

Applications using Proteinase K

• Preparation of DNA and RNA.
• Inactivation of RNase, DNase and enzymes.
• Isolation of genomic DNA.
• Isolation of RNA.

Concentration

20 mg/ml

Storage

at -20 °C for one year

Manual and datasheet

GE201 Proteinase K