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Proteinase K (20 mg/ml) – GE201-01

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Proteinase K (20 mg/ml)

Proteinase K is a nonspecific serine protease that will hydrolyze a variety of peptide bands. Proteinase K is active in a broad range of temperature and buffers. It cannot be inactivated by metal ions, chelating agents (e.g. EDTA), or detergents such as SDS.


Properties of Proteinase K

  • Active in a wide range of buffers and pH value.
  • Incubation temperature: 55-65 °C
  • Incubation time: 15 minutes to 48 hours; optimal incubation time: 2 hours
  • Included in most of our genomic DNA extraction kits and RNA extraction kits

Source

  • Purified from Tritirachium album

Applications using Proteinase K

  • Preparation of DNA and RNA.
  • Inactivation of RNase, DNase and enzymes.
  • Isolation of genomic DNA.
  • Isolation of RNA.

Concentration

20 mg/ml


Storage

at -20 °C for one year


Manual and datasheet

GE201 Proteinase K

Additional information

Format

1 ml

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