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DMT Enzyme ( Dpn I ) – GD111

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Product Description

DMT Enzyme

DMT, also known as DpnI, cuts the sequence mGATC (G is methylated) but does not cut the sequence GATC (G is not methylated). This enzyme cuts DNA prepared from most commonly used E.coli strains (dam+ strain), but does not cut PCR products.

Source

An E.coli strain that carries the cloned DMT enzyme gene from Diplococcus pneumoniae.

Unit Definition

One unit is the amount of enzyme required to completely digest 1 μg of pBR322 DNA (prepared from dam+ strain) in 50 μl of reaction mixture in 1 hour at 37°C.

Quality Control

Functional absence of endonucleases and exonucleases activities

Applications

In vitro site-directed mutagenesis, digestion of methylated DNA.

Concentration

10 units/μl

Storage

at -20 °C for two years

Manual and datasheet:

GD111 DMT Enzyme (DpnI)

Additional information

Format

200 u

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