DMT Enzyme ( Dpn I ) – GD111
DMT, also known as DpnI, cuts the sequence mGATC (G is methylated) but does not cut the sequence GATC (G is not methylated). This enzyme cuts DNA prepared from most commonly used E.coli strains (dam+ strain), but does not cut PCR products.
An E.coli strain that carries the cloned DMT enzyme gene from Diplococcus pneumoniae.
One unit is the amount of enzyme required to completely digest 1 μg of pBR322 DNA (prepared from dam+ strain) in 50 μl of reaction mixture in 1 hour at 37°C.
Functional absence of endonucleases and exonucleases activities
In vitro site-directed mutagenesis, digestion of methylated DNA.
at -20 °C for two years
Manual and datasheet:
GD111 DMT Enzyme (DpnI)
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