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DMT Enzyme ( Dpn I ) – GD111

Buy 1000 to 1800 units of DMT (DpnI) and receive an instant 7.5% discount.
Buy 2000 units or more and enjoy an instant 15% discount.
Buy 4000 units of DpnI
(20 packs) or more to obtain an instant 25% discount!

80.00$ CAD

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SKU: GD111 Categories: , Tags: , , Brand:

DMT Enzyme

DMT, also known as DpnI, cuts the sequence mGATC (G is methylated) but does not cut the sequence GATC (G is not methylated). This enzyme cuts DNA prepared from most commonly used E.coli strains (dam+ strain), but does not cut PCR products.

Source

An E.coli strain that carries the cloned DMT enzyme gene from Diplococcus pneumoniae.

Unit Definition

One unit is the amount of enzyme required to completely digest 1 μg of pBR322 DNA (prepared from dam+ strain) in 50 μl of reaction mixture in 1 hour at 37°C.

Quality Control

Functional absence of endonucleases and exonucleases activities

Applications

In vitro site-directed mutagenesis, digestion of methylated DNA.

Concentration

10 units/μl

Storage

at -20 °C for two years


Manual and datasheet

GD111 DMT Enzyme (DpnI)

Additional information

Format

200 u

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