Mouse VIP (Vasoactive Intestinal Peptide) ELISA Kit – E-EL-M1234

Mouse VIP (Vasoactive Intestinal Peptide) ELISA Kit

The Mouse VIP ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with Mouse VIP. During the reaction, Mouse VIP in the sample or standard competes with a fixed amount of Mouse VIP on the solid phase supporterfor sites on the Biotinylated Detection Ab specific to Mouse VIP. Excess conjugate and unbound sample or standard are washed from the plate, andAvidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse VIP in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: Mouse Vasoactive Intestinal Peptide

Abbreviation : VIP

Uniprot ID : P32648

Format : 96 rx

Reactivity : Mouse

ELISA configuration : Competitive

Sensitivity : 7.5 pg/ml

Detection Range : 12.5 – 800 pg/ml

Manual & Datasheet

More information about this Mouse VIP (Vasoactive Intestinal Peptide) ELISA Kit from Elabscience