Mouse TMED1 (Transmembrane Emp24 Domain Containing Protein 1) ELISA Kit – E-EL-M1196

Mouse TMED1 (Transmembrane Emp24 Domain Containing Protein 1) ELISA Kit

The Mouse TMED1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TMED1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse TMED1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TMED1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TMED1. You can calculate the concentration of Mouse TMED1 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Mouse Transmembrane Emp24 Domain Containing Protein 1

Abbreviation : TMED1

Uniprot ID : Q3V009

Format : 96 rx

Reactivity : Mouse

ELISA configuration : Sandwich

Sensitivity : 0.094 ng/ml

Detection Range : 0.156 – 10 ng/ml

Manual & Datasheet

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