Mouse CA1 (Carbonic Anhydrase I) ELISA Kit – E-EL-M0228

Mouse CA1 (Carbonic Anhydrase I) ELISA Kit

The Mouse CA1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CA1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for CA1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CA1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of CA1. You can calculate the concentration of CA1 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Mouse Carbonic Anhydrase I

Abbreviation : CA1

Uniprot ID : P13634

Format : 96 rx

Reactivity : Mouse

ELISA configuration : Sandwich

Sensitivity : 0.188 ng/ml

Detection Range : 0.313 – 20 ng/ml

Manual & Datasheet

More information about this Mouse CA1 (Carbonic Anhydrase I) ELISA Kit from Elabscience