Mouse BSP (Bone Sialoprotein) ELISA Kit – E-EL-M0765

Mouse BSP (Bone Sialoprotein) ELISA Kit

The Mouse BSP ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat BSP. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat BSP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BSP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat BSP. You can calculate the concentration of Rat BSP in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Mouse Bone Sialoprotein

Abbreviation : BSP

Uniprot ID : 

Format : 96 rx

Reactivity : Mouse

ELISA configuration : Sandwich

Sensitivity : 0.188 ng/ml

Detection Range : 0.313 – 20 ng/ml

Manual & Datasheet

More information about this Mouse BSP (Bone Sialoprotein) ELISA Kit from Elabscience