Mouse APO-A1 (Apolipoprotein A1) ELISA Kit – E-EL-M0130

Mouse APO-A1 (Apolipoprotein A1) ELISA Kit

The Mouse APO-A1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to APO-A1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for APO-A1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain APO-A1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of APO-A1. You can calculate the concentration of APO-A1 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Mouse Apolipoprotein A1

Abbreviation : APO-A1

Uniprot ID : Q00623

Format : 96 rx

Reactivity : Mouse

ELISA configuration : Sandwich

Sensitivity : 4.688 ng/ml

Detection Range : 7.813 – 500 ng/ml

Manual & Datasheet

More information about this Mouse APO-A1 (Apolipoprotein A1) ELISA Kit from Elabscience