Mouse APLN (Apelin) ELISA Kit – E-EL-M2422

Mouse APLN (Apelin) ELISA Kit

The Mouse APLN ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with Mouse APLN. During the reaction, Mouse APLN in the sample or standard competes with a fixed amount of Mouse APLN on the solid phase supporterfor sites on the Biotinylated Detection Ab specific to Mouse APLN. Excess conjugate and unbound sample or standard are washed from the plate, andAvidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse APLN in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: Mouse Apelin

Abbreviation : APLN

Uniprot ID : Q9R0R4

Format : 96 rx

Reactivity : Mouse

ELISA configuration : Competitive

Sensitivity : 75 pg/ml

Detection Range : 125 – 8000 pg/ml

Manual & Datasheet

More information about this Mouse APLN (Apelin) ELISA Kit from Elabscience