Mouse APAF1 (Apoptotic Peptidase Activating Factor 1) ELISA Kit – E-EL-M0143

Mouse APAF1 (Apoptotic Peptidase Activating Factor 1) ELISA Kit

The Mouse APAF1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to APAF1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for APAF1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain APAF1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of APAF1. You can calculate the concentration of APAF1 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Mouse Apoptotic Peptidase Activating Factor 1

Abbreviation : APAF1

Uniprot ID : O88879

Format : 96 rx

Reactivity : Mouse

ELISA configuration : Sandwich

Sensitivity : 4.688 pg/ml

Detection Range : 7.813 – 500 pg/ml

Manual & Datasheet

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