Mouse ALCAM (Activated Leukocyte Cell Adhesion Molecule) ELISA Kit – E-EL-M2456

Mouse ALCAM (Activated Leukocyte Cell Adhesion Molecule) ELISA Kit

The ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ALCAM. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse ALCAM and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ALCAM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ALCAM. You can calculate the concentration of Mouse ALCAM in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Mouse Activated Leukocyte Cell Adhesion Molecule

Abbreviation : ALCAM

Uniprot ID : Q61490

Format : 96 rx

Reactivity : Mouse

ELISA configuration : Sandwich

Sensitivity : 0.094 ng/ml

Detection Range : 0.156 – 10 ng/ml

Manual & Datasheet

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