Mouse 12-LO (Arachidonate 12-Lipoxygenase) ELISA Kit – E-EL-M1279

Mouse 12-LO (Arachidonate 12-Lipoxygenase) ELISA Kit

The Mouse 12-LO ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse 12-LO. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse 12-LO and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse 12-LO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse 12-LO. You can calculate the concentration of Mouse 12-LO in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Mouse Arachidonate 12-Lipoxygenase

Abbreviation : 12-LO

Uniprot ID : P39655

Format : 96 rx

Reactivity : Mouse

ELISA configuration : Sandwich

Sensitivity : 0.469 ng/ml

Detection Range : 0.781 – 50 ng/ml

Manual & Datasheet

More information about this Mouse 12-LO (Arachidonate 12-Lipoxygenase) ELISA Kit from Elabscience