Mouse α-CTx (Alpha Crosslaps) ELISA Kit – E-EL-M0361

Mouse α-CTx (Alpha Crosslaps) ELISA Kit

The Mouse α-CTx ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with α-CTx. During the reaction, Mouse α-CTx in the sample or standard competes with a fixed amount of α-CTx on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse α-CTx. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse α-CTx in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: Mouse Alpha Crosslaps

Abbreviation : α-CTx

Uniprot ID :

Format : 96 rx

Reactivity : Mouse

ELISA configuration : Competitive

Sensitivity : 46.875 pg/ml

Detection Range : 78.125 – 5000 pg/ml

Manual & Datasheet

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