Monkey F12 (Coagulation Factor XII) ELISA Kit – E-EL-MK0488

Monkey F12 (Coagulation Factor XII) ELISA Kit

The F12 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey F12. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Monkey F12 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey F12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey F12. You can calculate the concentration of Monkey F12 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Coagulation Factor Ⅻ

Abbreviation : F12

Uniprot ID :

Format : 96-well plate

Reactivity : Monkey

ELISA configuration : Sandwich

Sensitivity : 0.938 ng/ml

Detection Range : 1.563 – 100 ng/ml

Manual & Datasheet

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